: 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. for ValitaTiter Assays. 0 fmol/well FITC 200 µL in 96 wells. SpectraMax Plus 384 and 340PC 384 readers can accommodate standard 96-well, 384-well, and strip-well microplates. , Sigma cat. We obtained three HEK-293 cell lines from Clontech, each stably transfected with a different fluorescent protein. Ultraviolet (UV) measurements in microplates became possible when Molecular Devices introduced the first UV-capable microplate reader. Abstract and Figures. absorbance, fluorescence, and luminescence with the added functionality of. Cross-talk < 0. The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs),. SpectraMax Glo Steady-Luc Reporter Assay Kit, Molecular Devices (Explorer kit #R8352 or Bulk kit #R8353) Competitor kit: Steady-Glo Luciferase Assay System, Promega (Cat# E2510) SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903) Purified luciferase, Promega (Cat# E1701) The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. Detection modalities are shown in Table 1-1. With optimized reagents, option to operate the. Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. Primary Disinfectant (Cavicide, Warehouse, Cat # 79300360, or equivalent) 6. Molecular Devices i3x. Average StDev (mP) 1. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. Molecular Devices announced today the launch of the new SpectraMax ® ABS and ABS Plus Microplate Readers that cover a wide range of absorbance-based applications such as ELISAs, microbial growth, and protein quantitation and can be easily integrated into full robotic systems. Catalog Number: (10822-512) Supplier: Molecular Devices Description: Future-proof any laboratory with the SpectraMax® Paradigm® Multi-Mode Microplate Reader. 1 mL/well of media. Molecular Devices' microplate readers. Materials. MAXline Microplate Readers User’s Manual iii Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the MAXline Microplate Reader excluding the lamp assembly are warranted for aSpectraMax Microplate Readers. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. . . Four wash/rinse bottles are included and configurable for both 96- and 384. In . July 17, 2023. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. Published Dec 16, 2020 | Updated Dec 06, 2021. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. 40266. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Elisa Plate Readers and Microplate Readers at Biocompare. Standard features on the EMax® Plus Microplate Reader include the ability to measure both flat and round 96-well microplates, automatic lamp calibration prior to each reading (ensuring automatic measurements), powerful curve fitting protocols and statistical analysis tools, and integrated instrument control and data analysis with SoftMax® Pro. Read one sample or up to 384 in a. The concentration of total free thiols in the protein. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. 96-well and 384-well plates; Wavelength(s): 405, 450, 490, 560 and 600nm (Abs) Specifications/Features: Shaking, Temperature control, Injector. SOP Title: Use and. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Our specialized plate readers for absorbance, fluorescence, and luminescence detection provide optimal sensitivity. 2 nm Wavelength Bandpass 2. The drawing shows a standard microplate of 15 mm height covered with a lid. 96-well solid bottom, clear polystyrene microplates (Greiner cat. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. Microplate reader assays requiring infrared (IR) or red-shifted. #ID3-STD) L-Tryptophan (Sigma cat. ± 0. #N207) LAL Reagent Water (Lonza cat. Fluorescence Microplate Reader Comparison Chart. ) 20,000 10,000 30,000 40,000 50,000 60,000 70,000 80,000 0. When reading at wavelengths. 384 well polystyrene microplates have plate dimensions of 127. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. The FilterMax F5 can read 6, 12, 24, 48, 96, 384, 1536-well. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. The clear UltraVision FC 96-well microplate (FC_UltraVision (clear, 96)) showed the best performance of the tested microplates, with absorbance of 0. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. Microscopy. This reliable model allows for accurate wavelength detection in visible spectrum without the need for filters. Multimode Reader Comparison Chart. The Molecular Devices SpectraMax M2e Microplate Reader makes UV-Vis absorbance (200-1000 nm), fluorescence intensity (excitation 250-850 nm, emission 250-850 nm), chemiluminescence intensity (250-850 nm), and time-resolved fluorescence (50-1450 µsec, 200 µsec increments) measurements in 6, 12, 24, 48, 96, and 384 well microplates or in. It also integrates with the powerful SoftMax Pro Software for. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. Four wash/rinse bottles are included and configurable for both 96- and 384. Six wells of PBS were used as blanks for calculating the lower limit of detection (LLD). #M5e) Methods Microplate reader compatibility test. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Supplier: Molecular Devices. Place the microplate in the drawer. The procedure enables direct translation of any published equation. SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. 020 fmol 0. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. ZERO BIAS - scores, article reviews, protocol conditions and moreThe reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. 1 OD at 230 nm. BSA concentration (µg/mL) Average OD. 48 W x 12. adjust the reader. The UV-Vis SpectraMax 190 Microplate Reader for Quantification of DNA and endpoint, kinetic, and spectral scanning analyses. The EMax® Plus Microplate Reader is designed to be a versatile and robust microplate reader for research laboratories. This richly featured reader gives you the speed, convenience, and economy of monochromator-based wavelength selection for visible absorbance measurements between 340 nm and 850. This reader also makes use of a unique temperature-independent methodology for path length correction. #VAL004 or VAL014) 96-well assay plate • Human IgG for use as standard (e. is required for optimum performa nce with standard 96-well and 384-well format microplates for all top-read applications. 63. 3:40 . • 0. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and UV-visible absorbance. SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903). 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. At 5 seconds to read a 96-well endpoint assay, the EMax Microplate Reader has both the speed and performance to carry a heavy workload. #VAL004 or VAL014) 96-well assay plate • Human IgG for use as standard (e. Formats available are strip washers, full plate washers and combination washer-dispensers. When reading at wavelengths. All stand-alone instrument functions can be controlled using the software. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. adapter plate in drawer of Plate Reader with well A1 in position A1 of the drawer. 384 Awareness Technology ChroMate BioTek Synergy Mx. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. in space. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Microplate Readers. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. #VAL003 or VAL013) or ValitaTiter Plus (Molecular Devices cat. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. This reader utilizes Molecular Devices' patented PathCheck® Sensor to measure the depth (optical pathlength) of samples in a microplate. added to 96-well at 37 °C for 2 h, then the absorbance was measured at 450 nm by a. SpectraMax 190 and VersaMax readers can accommodate standard 96-well and strip-well microplates. #I2511)Introduction. ( a ) HCT116 cells expressing hyBRET-ERK were plated in 96-well plates and treated with serially diluted. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. 3. Reads standard cuvettes & 96-384 well plates from 190 to 1000 nm. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Affordable multi-mode microplate reader with . The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. In addition, the iD5 reader measures TRF and FP and. Minneapolis, MN, USA), according to the manufacturer's protocol. 96-well and 384-well plates; Wavelength(s): 405, 450, 490, 560 and 600nm (Abs). 1536-well: 0. Limit of Detection: < 20 attomol ATP per well Dynamic range > 9 orders of magnitude. Compact design Minimum volume and. Molecular Devices 1311 Orleans Drive Sunnyvale, California 94089 Telephone: +1-408. It includes both shaking and incubation functions, and is compatible with 96- well microplates. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. 6- to 1536-well plates 4 96- to 384-well plates 4 With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. #I2511) • Assay diluent – may be cell culture medium. from Molecular Devices are multi-detection microplate readers with dual-monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). #1402-9700) 384-well low volume solid black microplate (Corning cat. An industry-renowned instrument for monitoring GPCRs and ion channels. 2 OD at 200 nm; this decreased to 0. Here, we show that Molecular Devices microplate readers can easily and noninvasively measure fluorescent proteins in living cells. System setup and calibration are controlled from within SoftMax Pro Software. This instrument is ideal for measuring dual luciferase assays in both 96- and 384-well plates, and SoftMax Pro® software simplifies data collection and analysis with preconfigured protocols. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. Molecular Devices Corporation (the “Seller”) shall not be liable for any incidental or consequential damages. The SpectraMax ABS Plus reader was heated to 37ºC prior to setting up the assay. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. is required for optimum performance with standard 96-well and 384-well format microplates for all top-read applications. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. This reader's dual monochromators for. Outer wells are aligned for proper viewing with FLIPR. Molecular Devices VersaMax Microplate Reader Specifications. TempPlate semi-skirted 96-well PCR plate (USA Scientific cat. 3% in white 96- and. Retrieving. ThermoMax performs automatic mixing before and between readings to ensure reliable and precise. This can increase throughput and efficiency, thus saving time and space in the lab. In simplest terms, a plate reader measures and. The CellTiter-Glo assay from Promega uses luciferase. 2. Wren supernatant was transferred to a fresh 96-well microplate and absorbance recorded at 620 nm using a SpectraMax M3 (Molecular Devices) spectrophotometer. A multitude of modalities. 4. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl. ***Figure 1. 375 pM. Compact design Minimum volume and maximum throughput with 96- or 384-well microplate compatibility Powerful data analysis with SoftMax® Pro Software Optimal performance with validation tools Small but Mighty The IC design and microplate construct represents a major advancement to state-of-the-art CMOS devices for biological measurement, where the large majority are formed in single well constructs 12. F3/F5 Multi-Mode Microplate Readers. #3676). The system comes with a single plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well. Operates as a stand-alone reader or with input/output stackers and bottom reading optics. Step 3: Click “Settings” and a screen will appear (Figure 1). SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. . Fastest Read Time - 96-well plate: 20 sec; 384-well plate: 30 sec. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set ofMulti-mode microplate readers with fluorescence detection. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. 03 pM. The BioTek 800 TS absorbance reader is an affordable, high-quality microplate reader for assays in 6- to 384-well formats. fluorescence microplate reader from Molecular Devices. Next generation SpectraMax® absorbance microplate readers. 96-well microplate at 200 µL/well. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. Step 1: Turn on your microplate reader. The fluorescence intensity was captured with a SpectraMax Paradigm Multi-Mode Microplate Reader (Molecular Devices, LLC, San Jose, CA, USA) at 588 nm excitation and 633. Paradigm: Delta mP. 46Click the Read button in the SoftMax Proplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. Both COC microplates, the μCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. Use black microplates. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Incubate the plate at 37 °C for approximately one hour. Molecular Devices gmbH Tel. The SpectraDrop Micro-Volume Microplate offers the ability to use as little as 2˜µL samples with 24- or 64-well plates. 03 pM. This page contains the. Important things to consider when purchasing a multimode microplate reader. flat meniscus), but is approx 15 mL. Related Products: Low Binding Plates. 3. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 39. #W50-100) SpectraMax® ABS Plus Microplate Reader. For more sample throughput, both readers can be easily integrated into full roboticVisionMate 96 (Vial Rack Bottom Label 2D Reader) Ziath: Datapaq Express (Vial Rack Bottom Label 2D Reader). Accuris MR9600 SmartReader 96 Microplate Absorbance Reader. 69: 85. i3x. We would like to show you a description here but the site won’t allow us. SpectraMax ABS Plus reader utilizes Molecular Devices PathCheck Sensor- the only patented. Keywords: fluorescence polarization, trf, fret, igg. 1536-well: 0. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. SpectraMax® 340PC384 Microplate Reader for. For more great deals on Microplate readers by Molecular Devices, please browse our Molecular Devices Microplate readers page. It is user upgradeable and budget friendly, and comes with a brochure that provides detailed information on its features, specifications and applications. Vrla used the device to quantify the concentration of a small molecule produced by bacteria. The system is operated through the industry-leading SoftMax® Pro. The absorbance of the tested solutions was measured at 405 nm using a SpectraMax® ABS PLUS Absorbance Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA). 585The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. 98 VMax R2 = 0. plate height between 12–16 mm and 96-well “strip” plates Barcode libraries Interleaved 2 of 5, Code 128, EAN 128, Code. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. 5 mm and 15. The Perkin Elmer Wallac 1420. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. Built in near field communication (NFC). Detergent Compatible Bradford assay. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). 76 L x 85. Timothy Bolus, MPM, MBA, Compliance Program Manager. The SpectraMax ABS Plus Microplate Reader can run both cuvette-based and microplate based reader applications on the same instrument. 0 96 Well Plate. (12) Variants in ASPH cause exertional heat illness and are associated with malignant hyperthermia susceptibility Nature CommunicationsDecember 1, 2022 Yukari Endo, Linda Groom, Alper Celik, Natalia. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Our SpectraTest® ABS2, FL1, LM1,The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Page 11: Reader ComponentsBioTek Synergy HTX Microplate Reader - Absorbance Only Specifications. A preconfigured. The contents of the SpectraDrop Microplate kit depend on kit type. Bioz Stars score: 86/100, based on 1 PubMed citations. 1 pM 1536-well: 0. Pick the best speed, sensitivity, and price for your needs. The system provides automation for up to 50 microplates for easy walkaway automation. After 24 hours, cells were trypsinized and seeded at 100 μL/well into a 96-well plate starting at 30,000 cells/well with subsequent 2-fold dilutions. Since then, the microplate measurements of DNA, RNA, and proteins that this enabled have become very popular. Features. Can be integrated with Molecular Devices' StakMax® Microplate Stacker. 0 OD at. 03 pM 384-well: 0. We demonstrate that miniaturizing the assay in 96-well half area microplates, and using the flexible liquid transfer features of the FlexStation ® 3 Multi-Mode Microplate Reader allows rapid and reliable measurement of EC 50 values for agonists and IC 50 values for antagonists. The ThermoMax performs automatic mixing before a reading and between readings to ensure reliable and precise. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. The color touch screen provides a visual user interface, making programming fast and intuitive. SpectraMax iD5. 747. #iD5-STD) Methods ATP standard curve. A dependable washer is quiet, easy to clean and leaves no residue, preventing contamination and reducing background. explore more. 984. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate despite volume. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . Blank wells containing 50 µL culture medium only (n = 8) were also prepared. The VersaMax Absorbance Microplate Reader measures the optical density (OD) of samples at selected wavelengths in a number of reading modes. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence intensity assays. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. microplates or in a cuvette at a selected. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. NanoRacks added it to their line of commercial . #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. Pipet 100-200 µL of deionized water into each well of a clean 96-well microplate. and luminescence microplate reader validation DATA SHEET. Page 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. Molecular Devices 推出了FlexStation® 3 多功能读板机系统。FlexStation 3 把 SpectraMax M5e 和 8 道 / 16 道的移液 系统整合在一起成为体积小巧功能强大的 读板机系统。该整合系统不但为用户提供 了多功能检测平台，还增加了液体处理的 通量和提升了检测生化和细胞动. Despite its form, the Absorbance 96 delivers precise and accurate results at an affordable price. Molecular Devices is a leader in comprehensive compliance solutions with microplate detection systems. Keywords: fluorescence polarization, trf, fret, igg. 030 fmol Fluorescence Polarization (FP). Table 1: Categories of heating element design. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader. 1700 Online: Sales Offices. • SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. We would like to show you a description here but the site won’t allow us. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). Making it possible to perform kinetic analysis of solid. #ABS PLUS) Methods. The screw remains inside the instrument. ValitaTiter Assays Setup Guide 2601A October 2023. 4 nm Microplate Capacity Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates Incubation Temperature control range from Ambient +4º to 50ºC Temperature variation ± 0. In addition, the iD5 reader measures TRF and FP and. It is a UV/Vis spectrophotometer/microwell reader with built-in cuvette port and microplate drawer. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical. Running both standard spectrophotometer and microplate. Compare plate readers SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Optical absorbance and fluorescence were measured on a SpectraMax M5 Multi-Mode microplate reader (Molecular Devices, San Jose, CA). Narrow bandwidths deliver increased accuracy and enhanced peak resolution. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Equipped with scanning, shaking and kinetics modules. Listing: #4772697. 96-well plates: V-bottom: 20-30 µl U-bottom: 30-40 µl flat-bottom: 50-60 µl 384-well plates: approximately 20 µl Recommended ArticlesA method is described for the routine determination of the rate of colorimetric enzyme reactions using a 96-well microtiter plate reader commonly used in. +49-89/96-05-88-0 fax +49-89/9-62-02-34-5 Japan molecular devices Japan, osaka. 5. 0 H x 60. Plates can put in the drawer both in landscape and portrait positions. 2 OD at 200 nm; this decreased to 0. SpectraMax iD3 Multi-Mode Microplate Reader (Molecular Devices cat. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurements in the. i3x Multi-Mode Microplate Reader. Click on the crosshair and move it so that it is centered over the well image. These models can no longer be repaired. 2600A October 2023. SpectraDrop High-Throughput Screening (HTS) Kit. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. 7°C (gradient heating design). 1. VANTAstar™ Flexible Multi-mode Microplate Reader. I have used Molecular Devices’ Vmax 96-well microplate readers since about the time of their inception in the mid to late-1980s. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. #W50-100). In addition, SoftMax Pro Software Molecular Devices SpectraMax ® Paradigm ® Multi-Mode Microplate Detection Platform is a user upgradeable microplate reader that allows for real-time system configuration by the user in less than two minutes. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. While "plate reader" usually refers to the devices described. When the user applies a set temperature of 37°C, the top is heated to 37. The Gemini EM Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Geoffrey Vrla, a molecular biology graduate student at Princeton, found Tecan's Infinite 200 Pro Microplate Reader to be a versatile and accurate instrument for quantifying fluorescent proteins expressed in bacteria. Published Dec 16, 2020 | Updated Dec 16, 2020. 00 Read more; Molecular Devices Absorbance Microplate Reader VersaMax Molecular Devices EMax Microplate Readertriple-mode cuvette port and 6-well to 384-well microplate reading capability. Prior to the reading of each plate, the microplate reader was “blanked” with a plate containing 0. 63. Insert the filled microplate into the drawer, matching well A1 with position A1. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. A 1:10 serial dilution of ATP was prepared in medium. Flexible Microplate Format The GEMINI system allows you to select from 6-, 12-, 24-, 48-, 96- and 384-well microplate formats for your fluorometric measurements. By far the most compact reader on the market, the footprint of the reader is almost as small as the plate itself. Plate type 96-well standard clear bottom: Timing Total run time: 01:40:00: Interval: 00:01:00 More settings : Carriage speed: Slow:The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Read one sample or up to 384 in a single plate eead using any standard cuvette, or 96-or 384-well microplate. 5°C at 37°C. SpectraMax Mini Multi-Mode Microplate Reader Afordable multi-mode microplate reader with best-in-class data analysis software Perform your favorite applications including. After 5, 7, 11, and 13 days. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. 8 Pages. Instrument settings can beAbsorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. Read More. The Molecular Devices Versamax Microplate Reader INCLUDED:. " A versatile UV/VIS spectrophotometer for both 96- and 384-well microplates as well as cuvette. Standard features on the EMax® Plus Microplate Reader include the ability to measure both flat and round 96-well microplates, automatic lamp calibration prior to each reading (ensuring automatic measurements), powerful curve fitting protocols and statistical analysis tools, and integrated instrument control and data analysis with SoftMax® Pro. to each well. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. 2 2601A ValitaTiter. +49-89/96-05-88-0 Fax +49-89/9-62-02-34-5 Japan. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile instrument that can measure absorbance, fluorescence and luminescence in microplates. Both COC microplates, the µCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. validation, maintenance, and repair documentation. g. A polyclonal antibody specific to mouse/rat IL-22 was pre-coated onto a 96-well microplate. Use standard plate unless you see your type listed. Once the assay runs its course, the final absorbance readout will take place on a SpectraMax® iD5 Multi-Mode Microplate Reader. This unlocks the transport slide. changes in OD 600 were measured by periodically transferring 100-µL aliquots of each bacterial culture to a single well of a 96-well microplate and measuring absorbance at 600 nm using the SpectraMax iD3 reader and SoftMax Pro Software. BMG LABTECH. Integrated stacker option. Optimized optical path - The reader’s unique quartz fiber optic system minimizes stray light for precise readings across a microplate. Industry standard, 96-well plates are. If the best wavelengths are unknown, the Gemini EM reader will find. 05. While they play an important role in generating data from a wide range of. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Up to 70% savings in overall assay time can be gained when using the Cell Cytotoxicity assay (Figure 1). SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. For FlexStation I/II/3 plate readers, what are the dead-volumes for the compound plate? The FlexStation plate readers assume a 1 mm pipette height from the bottom of the well when aspirating from a compound source plate. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. In combination with the SpectraMax Mini Multi-Mode Microplate Reader, it enables increased throughput and detection of DNA as low as 2 ng/μL using just a few microliters of sample. Model:. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. 19 H mm that meet microplate industry standards. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. The SpectraMax Plus 384 Microplate Reader is a good choice for running red wine phenolics assay described by Heredia et al. University of Reading Simon Lydford: Molecular Devices (UK) Ltd.